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adamts 4  (Proteintech)


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    Proteintech adamts 4
    The Composite Hydrogel Substrate Fosters a Pro-Anabolic and Anti-Catabolic Microenvironment for Adherent Nucleus Pulposus Cells. ( A ) Schematic of the in vitro culture model where NPCs are seeded on different hydrogel formulations. ( B ) Histochemical staining for matrix deposition by NPCs cultured on different hydrogels, including Alcian Blue (top row) and Toluidine Blue (bottom row) staining. Scale bar: 200 μm. ( C ) Western blot analysis of anabolic (COL-2, ACAN) and catabolic (MMP-13, <t>ADAMTS-4)</t> protein markers in NPCs cultured on different hydrogel substrates. GAPDH served as a loading control. ( D ) Dot plot visualization of the relative protein expression levels from Fig. 3E The size and color intensity of each dot correspond to the expression level. (E) Western blot analysis of key proteins in the integrin-mediated signaling pathway (p-FAK, p-ERK, p-p38). ( F ) Dot plot visualization of the relative protein expression levels from Fig. 3E. ( G ) Western blot analysis of anabolic and catabolic markers in the presence of an integrin α2β1 blocking antibody (iα2β1). ( H ) Dot plot visualization of the relative protein expression levels from Fig. 3G. ( I ) Representative immunofluorescence images for Collagen II (green), MMP-13 (red), and Aggrecan (green) in NPCs cultured on different hydrogels. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. ( J ) Quantitative analysis of the relative fluorescence intensity for COL-2, MMP-13, and ACAN from the images in Fig. 3G. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant
    Adamts 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adamts+4/ADAMTS4+Fusion+Protein/pmc13020143-325-23-35
    Average 94 stars, based on 11 article reviews
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    Images

    1) Product Images from "Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration"

    Article Title: Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-026-04211-w

    The Composite Hydrogel Substrate Fosters a Pro-Anabolic and Anti-Catabolic Microenvironment for Adherent Nucleus Pulposus Cells. ( A ) Schematic of the in vitro culture model where NPCs are seeded on different hydrogel formulations. ( B ) Histochemical staining for matrix deposition by NPCs cultured on different hydrogels, including Alcian Blue (top row) and Toluidine Blue (bottom row) staining. Scale bar: 200 μm. ( C ) Western blot analysis of anabolic (COL-2, ACAN) and catabolic (MMP-13, ADAMTS-4) protein markers in NPCs cultured on different hydrogel substrates. GAPDH served as a loading control. ( D ) Dot plot visualization of the relative protein expression levels from Fig. 3E The size and color intensity of each dot correspond to the expression level. (E) Western blot analysis of key proteins in the integrin-mediated signaling pathway (p-FAK, p-ERK, p-p38). ( F ) Dot plot visualization of the relative protein expression levels from Fig. 3E. ( G ) Western blot analysis of anabolic and catabolic markers in the presence of an integrin α2β1 blocking antibody (iα2β1). ( H ) Dot plot visualization of the relative protein expression levels from Fig. 3G. ( I ) Representative immunofluorescence images for Collagen II (green), MMP-13 (red), and Aggrecan (green) in NPCs cultured on different hydrogels. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. ( J ) Quantitative analysis of the relative fluorescence intensity for COL-2, MMP-13, and ACAN from the images in Fig. 3G. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant
    Figure Legend Snippet: The Composite Hydrogel Substrate Fosters a Pro-Anabolic and Anti-Catabolic Microenvironment for Adherent Nucleus Pulposus Cells. ( A ) Schematic of the in vitro culture model where NPCs are seeded on different hydrogel formulations. ( B ) Histochemical staining for matrix deposition by NPCs cultured on different hydrogels, including Alcian Blue (top row) and Toluidine Blue (bottom row) staining. Scale bar: 200 μm. ( C ) Western blot analysis of anabolic (COL-2, ACAN) and catabolic (MMP-13, ADAMTS-4) protein markers in NPCs cultured on different hydrogel substrates. GAPDH served as a loading control. ( D ) Dot plot visualization of the relative protein expression levels from Fig. 3E The size and color intensity of each dot correspond to the expression level. (E) Western blot analysis of key proteins in the integrin-mediated signaling pathway (p-FAK, p-ERK, p-p38). ( F ) Dot plot visualization of the relative protein expression levels from Fig. 3E. ( G ) Western blot analysis of anabolic and catabolic markers in the presence of an integrin α2β1 blocking antibody (iα2β1). ( H ) Dot plot visualization of the relative protein expression levels from Fig. 3G. ( I ) Representative immunofluorescence images for Collagen II (green), MMP-13 (red), and Aggrecan (green) in NPCs cultured on different hydrogels. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. ( J ) Quantitative analysis of the relative fluorescence intensity for COL-2, MMP-13, and ACAN from the images in Fig. 3G. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant

    Techniques Used: In Vitro, Staining, Cell Culture, Western Blot, Control, Expressing, Blocking Assay, Immunofluorescence, Fluorescence

    Related Articles

    Incubation:

    Article Title: Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration
    Article Snippet: Equal amounts of protein (20 μg) per sample were separated by 8–12% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, California, USA). .. Membranes were blocked with 5% non-fat milk in TBST for 1 h and incubated overnight at 4 °C with primary antibodies against: ACAN, ADAMTS-4, COL-II, MMP-13, GPX4, FHC, p-p53, p53, GLS2, BCL2L11, and CASP7 (1:1000; ProteinTech, Wuhan, China). .. After washing, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3000; ProteinTech, Wuhan, China) for 1.5 h at room temperature.

    Article Title: Angiopoietin-2 Promotes Mechanical Stress-induced Extracellular Matrix Degradation in Annulus Fibrosus Via the HIF-1α/NF-κB Signaling Pathway.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense diluted with Tris-buffered saline containing 0.1% Tween 20 for 1 h and incubated with antibodies against Col2a1, MMP-13, ADAMTS-4, HIF-1α, β-actin (purchased from Abcam, Cambridge, MA, USA), p65, IKB (purchased from Proteintech, Wuhan, China) overnight at 4 C. The strips were then incubated with a secondary antibody (purchased from ABclonal, Wuhan, China) at room temperature for 1 h. An ultrasensitive electrochemiluminescence kit was used to detect the bands, and software was used to image and analyze the bands. ..

    Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.
    Article Snippet: .. Next, the membranes were incubated overnight, at 4°C with the following antibodies: Nrf2 (CSB-PA003481; Cusabio, Wuhan, China), aggrecan (13880-1-AP; Proteintech), COL2A1 (MA512789; Thermo Fisher), ADAMTS-5 (ab41037; Abcam, Cambridge, USA), MMP13 (CSB-PA07029A0Rb; Cusabio), p-p65 (ab194726 for human; Abcam; CSB-PA000582 for mouse; Cusabio), p65 (10745-1- AP for human; 80979-1-RR for mouse; Proteintech), HMGB1 (CSBPA01604A0Rb; Cusabio), C-caspase-3 (ab32042; Abcam), procaspase-3 (ab32150; Abcam), BAX (50599-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), ADAMTS-4 (11865-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), actin (66009-1-Ig, Proteintech) and GAPDH (60004-1-Ig; Proteintech), followed by incubation with HRP-conjugated secondary antibody (SA00001-1, SA00001-2; Proteintech) for 1 h at room temperature. .. The blot signals were then visualized using ECL Substrates (Merck Millipore, Billerica, USA).

    Article Title: Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration.
    Article Snippet: Equal amounts of protein (20 μg) per sample were separated by 8–12% SDS- PAGE and transferred to a PVDF membrane (Bio-Rad, California, USA). .. Membranes were blocked with 5% non-fat milk in TBST for 1 hour and incubated overnight at 4 °C with primary antibodies against: ACAN, ADAMTS-4, COL-II, MMP-13, GPX4, FHC, p-p53, p53, GLS2, BCL2L11, and CASP7 (1:1000; ProteinTech, Wuhan, China). .. After washing, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3000; ProteinTech, Wuhan, China) for 1.5 hours at room temperature.

    Saline:

    Article Title: Angiopoietin-2 Promotes Mechanical Stress-induced Extracellular Matrix Degradation in Annulus Fibrosus Via the HIF-1α/NF-κB Signaling Pathway.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense diluted with Tris-buffered saline containing 0.1% Tween 20 for 1 h and incubated with antibodies against Col2a1, MMP-13, ADAMTS-4, HIF-1α, β-actin (purchased from Abcam, Cambridge, MA, USA), p65, IKB (purchased from Proteintech, Wuhan, China) overnight at 4 C. The strips were then incubated with a secondary antibody (purchased from ABclonal, Wuhan, China) at room temperature for 1 h. An ultrasensitive electrochemiluminescence kit was used to detect the bands, and software was used to image and analyze the bands. ..

    Electrochemiluminescence:

    Article Title: Angiopoietin-2 Promotes Mechanical Stress-induced Extracellular Matrix Degradation in Annulus Fibrosus Via the HIF-1α/NF-κB Signaling Pathway.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense diluted with Tris-buffered saline containing 0.1% Tween 20 for 1 h and incubated with antibodies against Col2a1, MMP-13, ADAMTS-4, HIF-1α, β-actin (purchased from Abcam, Cambridge, MA, USA), p65, IKB (purchased from Proteintech, Wuhan, China) overnight at 4 C. The strips were then incubated with a secondary antibody (purchased from ABclonal, Wuhan, China) at room temperature for 1 h. An ultrasensitive electrochemiluminescence kit was used to detect the bands, and software was used to image and analyze the bands. ..

    Software:

    Article Title: Angiopoietin-2 Promotes Mechanical Stress-induced Extracellular Matrix Degradation in Annulus Fibrosus Via the HIF-1α/NF-κB Signaling Pathway.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense diluted with Tris-buffered saline containing 0.1% Tween 20 for 1 h and incubated with antibodies against Col2a1, MMP-13, ADAMTS-4, HIF-1α, β-actin (purchased from Abcam, Cambridge, MA, USA), p65, IKB (purchased from Proteintech, Wuhan, China) overnight at 4 C. The strips were then incubated with a secondary antibody (purchased from ABclonal, Wuhan, China) at room temperature for 1 h. An ultrasensitive electrochemiluminescence kit was used to detect the bands, and software was used to image and analyze the bands. ..



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    The Composite Hydrogel Substrate Fosters a Pro-Anabolic and Anti-Catabolic Microenvironment for Adherent Nucleus Pulposus Cells. ( A ) Schematic of the in vitro culture model where NPCs are seeded on different hydrogel formulations. ( B ) Histochemical staining for matrix deposition by NPCs cultured on different hydrogels, including Alcian Blue (top row) and Toluidine Blue (bottom row) staining. Scale bar: 200 μm. ( C ) Western blot analysis of anabolic (COL-2, ACAN) and catabolic (MMP-13, ADAMTS-4) protein markers in NPCs cultured on different hydrogel substrates. GAPDH served as a loading control. ( D ) Dot plot visualization of the relative protein expression levels from Fig. 3E The size and color intensity of each dot correspond to the expression level. (E) Western blot analysis of key proteins in the integrin-mediated signaling pathway (p-FAK, p-ERK, p-p38). ( F ) Dot plot visualization of the relative protein expression levels from Fig. 3E. ( G ) Western blot analysis of anabolic and catabolic markers in the presence of an integrin α2β1 blocking antibody (iα2β1). ( H ) Dot plot visualization of the relative protein expression levels from Fig. 3G. ( I ) Representative immunofluorescence images for Collagen II (green), MMP-13 (red), and Aggrecan (green) in NPCs cultured on different hydrogels. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. ( J ) Quantitative analysis of the relative fluorescence intensity for COL-2, MMP-13, and ACAN from the images in Fig. 3G. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant

    Journal: Journal of Nanobiotechnology

    Article Title: Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration

    doi: 10.1186/s12951-026-04211-w

    Figure Lengend Snippet: The Composite Hydrogel Substrate Fosters a Pro-Anabolic and Anti-Catabolic Microenvironment for Adherent Nucleus Pulposus Cells. ( A ) Schematic of the in vitro culture model where NPCs are seeded on different hydrogel formulations. ( B ) Histochemical staining for matrix deposition by NPCs cultured on different hydrogels, including Alcian Blue (top row) and Toluidine Blue (bottom row) staining. Scale bar: 200 μm. ( C ) Western blot analysis of anabolic (COL-2, ACAN) and catabolic (MMP-13, ADAMTS-4) protein markers in NPCs cultured on different hydrogel substrates. GAPDH served as a loading control. ( D ) Dot plot visualization of the relative protein expression levels from Fig. 3E The size and color intensity of each dot correspond to the expression level. (E) Western blot analysis of key proteins in the integrin-mediated signaling pathway (p-FAK, p-ERK, p-p38). ( F ) Dot plot visualization of the relative protein expression levels from Fig. 3E. ( G ) Western blot analysis of anabolic and catabolic markers in the presence of an integrin α2β1 blocking antibody (iα2β1). ( H ) Dot plot visualization of the relative protein expression levels from Fig. 3G. ( I ) Representative immunofluorescence images for Collagen II (green), MMP-13 (red), and Aggrecan (green) in NPCs cultured on different hydrogels. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. ( J ) Quantitative analysis of the relative fluorescence intensity for COL-2, MMP-13, and ACAN from the images in Fig. 3G. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant

    Article Snippet: Membranes were blocked with 5% non-fat milk in TBST for 1 h and incubated overnight at 4 °C with primary antibodies against: ACAN, ADAMTS-4, COL-II, MMP-13, GPX4, FHC, p-p53, p53, GLS2, BCL2L11, and CASP7 (1:1000; ProteinTech, Wuhan, China).

    Techniques: In Vitro, Staining, Cell Culture, Western Blot, Control, Expressing, Blocking Assay, Immunofluorescence, Fluorescence